MACROPHAGE-LYMPHOCYTE CLUSTERS IN THE IMMUNE-RESPONSE TO SOLUBLE-PROTEIN ANTIGEN INVITRO .7. GENETICALLY RESTRICTED AND NON-RESTRICTED PHYSICAL INTERACTIONS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (4) , 1608-1613
Abstract
The genetic restrictions on physical interactions between macrophages and central lymphocytes and between central and peripheral lymphocytes were assessed in antigen[Ag]-specific macrophage-lymphocyte clusters with respect to I-region differences of inbred strains 2 and 13 guinea pigs. When using lymphocytes from guinea pigs immunized with DNP-OVA [dinitrophenylated ovalbumin] or DNP-GL [DNP-copolymer of glutamic acid and lysine] in CFA, the antigen-specific interaction between central lymphocyte and macrophage requires that both cells be derived from animals syngeneic at the I-region of the major histocompatibility complex (MHC). In studies using Ag, the response to which is under the control of MHC-linked Ir genes, macrophages from the responder, but not from the nonresponder parental strain support cluster formation with responder .times. nonresponder F1(2 .times. 13) T cells. The physical interactions between central and peripheral T [thymus-derived] lymphocytes are not restricted by the I-region of the MHC and the peripheral lymphocyte need not be from an animal immune to the Ag used to drive macrophage central lymphocyte interactions.