Sequence analysis and biochemical characterisation of chromosomal CAV-1 (Aeromonas caviae), the parental cephalosporinase of plasmid-mediated AmpC ‘FOX’ cluster
Open Access
- 1 May 2003
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 222 (1) , 93-98
- https://doi.org/10.1016/s0378-1097(03)00253-2
Abstract
Aeromonas caviae CIP 74.32 was resistant to amoxicillin, ticarcillin and cephalothin, and susceptible to cefoxitin, cefotaxime, ceftazidime, aztreonam and imipenem. This strain produced a cephalosporinase (pI 7.2) and an oxacillinase (pI 8.5). The cephalosporinase gene cav-1 was cloned and sequenced. Unlike A. caviae donor, Escherichia coli pNCE50 transformant producing CAV-1 β-lactamase was resistant to cefoxitin. The deduced protein sequence CAV-1 contained 382 amino acids, and shared >96% homology with FOX-1 to FOX-5 cephalosporinase. CAV-1 presented only two amino acid substitutions (Thr270Ser and Arg271Ala) with FOX-1. CAV-1 is the chromosomal putative ancestor of the FOX family, a cluster of class C/group 1 plasmidic cephalosporinases spreading in Klebsiella and E. coli clinical isolates via conjugative plasmids.Keywords
This publication has 23 references indexed in Scilit:
- Secreted enzymes of AeromonasFEMS Microbiology Letters, 2006
- Characterization of a Novel Plasmid-Mediated Cephalosporinase (CMY-9) and Its Genetic Environment in anEscherichia coliClinical IsolateAntimicrobial Agents and Chemotherapy, 2002
- Cloning and Biochemical Characterization of FOX-5, an AmpC-Type Plasmid-Encoded β-Lactamase from a New York City Klebsiella pneumoniae Clinical IsolateAntimicrobial Agents and Chemotherapy, 2001
- High-Resolution Genotypic Analysis of the Genus Aeromonas by AFLP FingerprintingInternational Journal of Systematic and Evolutionary Microbiology, 1996
- Distribution ofAeromonasphenospecies and genospecies among strains isolated from water, foods or from human clinical samplesEpidemiology and Infection, 1995
- Sequence analysis of two chromosomally mediated inducible β-lactamases from Aeromonas sobria, strain 163a, one a class D penicillinase, the other an AmpC cephalosporinaseJournal of Antimicrobial Chemotherapy, 1995
- Phenotypic characteristics of the three hybridization groups of Aeromonas hydrophila complex isolated from different sourcesJournal of Applied Bacteriology, 1994
- The pathogenicity of Aeromonas strains relative to genospecies and phenospecies identificationFEMS Microbiology Letters, 1991
- A Taxonomic Study of the Aeromonas hydrophila-Aeromonas punctata GroupJournal of General Microbiology, 1976
- Computerized microacidimetric determination of β lactamase Michaelis—Menten constantsFEBS Letters, 1973