Siderocytes in mammalian blood

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Abstract
When citrated blood (man, cat, dog) was stored, siderocytes (erythrocytes with Fe granuels stainable by ferricyanide) appeared in 24 hrs. (5-10%) with simultaneous rise in plasma Fe and bile pigments. At 4[degree]C, siderocytosis was delayed 4 days. Glucose delayed onset of siderocytosis 14 days. Oxygenation produced irregular cyclic rise and fall of siderocytes. CO reduced siderocyte formation. Addition of 500 [gamma] % acetyl phenylhydrazine (APH) produced 20% siderocytosis in 2 hrs with simultaneous loss of 5% of Hb, although APH did not destroy Hb in laked blood. A further addition of 500 [gamma] % APH was without further effect. Following APH, erythrocytes extruded siderotic granules which were ingested by polymorphonucleocytes, and mononucleocytes ingested siderocytes. In a normal [male], 0.1 g. APH/day for 5 days lowered erythrocyte count from 5 million-2.7 million with simultaneous rise of siderocyte count to 11% and urinary sidero-sis which continued 4 days after termination of APH. Siderocytes are considered as effete erythrocytes, in which the siderotic granules are derived from a catabolic fraction of erythrocyte iron which is closely associated with the easily split blood Fe fraction. Siderocyte counts are suggested for the clinical evaluation of hemolytic processes.

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