INDUCTION AND INHIBITION OF α-GLUCOSIDASE SYNTHESIS IN CANDIDA STELLATOIDEA

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Abstract
C. stella-toidea grown in glucose broth could not ferment maltose immediately upon presentration of this disaccharide. Capability to use maltose developed in the absence of growth within a few hours subsequent to exposure to this sugar. Minimal nutritional requirements for expression of alpha-glucosidase activity were inducer and arginine. Development and retention of alpha-glucosidase were suppressed by glucose. Induced synthesis of enzyme was inhibited by 10-4 [image] 2,4-dinitrophenol and 10-4 [image] sodium azide but not by 2 [mu]g/ml nystatin or pimaricin. Unadapted yeasts, subcultured in medium containing both maltose and glucose, proliferated exponentially until the monosaccharide was depleted; they then entered the stationary phase. After 3-5 hours, the cells adapted the maltose and resumed growth. Studies using cell-free preparations of induced and uninduced yeasts demonstrated that presence of the enzyme alpha-glucosidase was dependent upon availability of maltose or dextrin in the medium.