Staining Pollen Tubes in the Style; Cotton BlueversusCarmine for General Use
- 1 January 1967
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 42 (2) , 81-85
- https://doi.org/10.3109/10520296709114986
Abstract
Pollen tubes passing through the style are fixed in acetic-alcohol (1 : 1) at 60 C for 30 min; softened by boiling in water 5–30 min; cleared in lactic acid at 60 C, 30 min; stained in lactophenol-cotton blue (1%) at 60 C, 15–30 min; and mounted in lactic acid. This procedure was found to be more generally applicable to angiosperms than carmine, applied in propionic-lactic acid mixtures. It has been tested on 36 species, including 4 monocotyledons and 32 dicotyledons.This publication has 15 references indexed in Scilit:
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