Nucleotide sequence of cloned complementary deoxyribonucleic acid for the .alpha. subunit of bovine pituitary glycoprotein hormones

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Abstract
Recombinant DNA plasmids containing sequences coding for the .alpha. subunit of the bovine pituitary glycoprotein hormones were isolated. The nucleotide sequences of 3 different c[complementary]DNA clones were determined. The largest .alpha.-subunit cDNA clone was found to contain 713 bases including 77 nucleotides from the 5''-untranslated region, 72 nucleotides coding for a precursor segment, 288 nucleotides coding for the mature .alpha. subunit and 276 nucleotides from the 3''-untranslated region of the mRNA followed by a poly(A) segment. This cDNA likely represents most of the bovine .alpha.-subunit mRNA sequence. Nucleotide sequences were obtained from the cDNA inserts of 2 other .alpha.-subunit clones, and several differences among the 3 cDNA sequences were detected. These differences in nucleotide sequence may represent either individual variation in genomic sequence or cloning artifacts. Comparison of the bovine .alpha.-subunit cDNA sequence to the sequences of human, rat and mouse .alpha.-subunit cDNA reveals that the bovine sequence has greater than 70% homology with the other cDNA. The cloned .alpha.-subunit cDNA should provide a useful probe for further studies of the structure and expression of this interesting gene.

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