Abscopal Suppression of Bone Marrow Erythropoiesis

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Abstract
Abscopal responses of hemopoietic tissue, which were noted in preliminary studies of mice receiving partial-body X irradiation were studied. One hind leg of CF-1 female mice received 1000, 5000 or 10,000 rad of X radiation. A persistent shift from medullary to splenic erythropoiesis preventing anemia in mice receiving 5000 or 10,000 rad was found. Splenectomy prior to 5000-rad irradiation resulted in anemia, which was not ameliorated by exposure to intermittent hypoxia. Despite evidence for increased levels of erythropoietin in the animals, namely, a reticulocytosis and increased erythrocyte radioiron incorporation, both 59Fe uptake and erythroblast counts in shielded marrow remained below normal. A suppression of the growth (50-90%) of marrow stromal colonies (MSC) from bone marrow aspirates of the shielded and irradiated femoral marrow at 1 mo. and at least 20% depression of MSC at 1 yr, with each dose was found. High doses of X radiation to 1 leg of mice caused prolonged suppression of medullary erythropoiesis with splenic compensation to prevent anemia, splenectomy, anemia, and hypoxia prevented the severe abscopal depression of medullary erythropoiesis and suppressed medullary erythropoiesis with decreased growth of MSC suggested a change in the hemopoietic microenvironment of the bone marrow.