A massively parallel PicoTiterPlate™ based platform for discrete picoliter‐scale polymerase chain reactions
- 6 November 2003
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 24 (21) , 3769-3777
- https://doi.org/10.1002/elps.200305646
Abstract
We demonstrate successful, simultaneous polymerase chain reaction (PCR) amplification of up to 300 000 discrete reactions in a novel platform, the PicoTiterPlate™. In addition to elevated throughput, the PicoTiterPlate™ based amplifications (PTPCR) can be performed in extremely small volumes: individual reactions volumes are as low as 39.5 pL, with a total 15.3 μL reaction volume for the entire PicoTiterPlate™. The bulk PTPCR product can be recovered and assayed with real-time PCR, or discrete PTPCR products can be driven to solid supports, enabling downstream applications such as translation/transcription or sequencing.Keywords
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