Improved radioimmunoassay of urinary estriol.
Open Access
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (1) , 99-102
- https://doi.org/10.1093/clinchem/25.1.99
Abstract
We report a rapid double-antibody radioimmunoassay for urinary estriol. Advantages over other current methods include: (a) 30-min hydrolysis; (b) total incubation time, 55 min; (c) assay unaffected by urinary glucose; (d) no degradation of estriol evident during hydrolysis; (e) superior (85%) analytical recovery of estriol conjugates; (f) linear standard curve by logit-log extrapolation; (g) good correlation (r = 0.83) with total estrogen determination by a generally accepted colorimetric method; (h) only 20 muL of urine required; and (i) the detection range is 1.9 to 100.5 mg/24-h urine.This publication has 7 references indexed in Scilit:
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