Ultrastructural Characterization of Herpes Simplex Virus Type 1 (Strain 17) Temperature-sensitive Mutants

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Abstract
The ultrastructural features with wild-type and temperature sensitive (ts) mutant infection at non-permissive temperature was reported for each of 18 herpes simplex virus type 1 (HSV-1) (strain 17) ts mutants. The mutants were classified by their ability to induce nucleocapsid-related structures: class I failed to induce any, class II induced empty and partially filled structures while class III induced all of the identified structures including those containing an electron-dense core. The time when expression of the ts lesion blocked virus replication was estimated for most mutants; this allows mutant gene expression and the resulting ultrastructural features to be correlated with the sequence of virus replicative events. Three ts mutant-triggered features not previously described in HSV-1-infected [hamster kidney BHK-21] cells were also recognized: a modification of rough endoplasmic reticulum, intranuclear accumulation of enveloped virus particles and cytoplasmic accumulation of novel doughnut-shaped particles having a concentric double ring appearance in thin section.