A CYTOGENETIC STUDY OF THE X-RAY INDUCED CAULIFLOWER-HEAD AND SINGLE-LEAF MUTATION INMEDICAGO SATIVAL

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Abstract
Data obtained from X2, X3, and X4 segregation ratios demonstrate that two closely associated characteristics, cauliflower-head and single-leaf are determined by a single recessive gene inherited tetrasomically. Chromosome counts revealed a trisomic tetraploid with 31 chromosomes, the normal complement (2n=32), and mutants showing an unstable condition. It is suggested that this chromosomal variation could have been induced by X-irradiation or it may have been transmitted from one of the parents. The trisomic genotype does not affect the phenotype of the mutant The results are discussed in connection with pollen X-irradiation and the polyploid nature of M. sativa L. (2n=32).
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