ELECTROPHORETIC BEHAVIOR AND CRYSTAL VIOLET ADSORPTION CAPACITY OF RIBONUCLEASE TREATED BACTERIAL CELLS

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Abstract
The treatment of Gram-negative or Gram-posi-tive bacteria with ribonuclease caused a quantitative decrease in the adsorption of crystal violet. Small but significant decreases in electrophoretic mobility were found in enzyme-treated cells of 13 of the 14 spp. studied. The pH at which electrophoretic comparisons were made was found to be an important factor in detecting cell surface changes due to ribonuclease treatment. Decrease in dye adsorption and electrical charge also occurred in cells depleted of nucleic acids by incubation in the absence of added nutrients. These findings are discussed briefly in relation to the location of ribonucleic acids within the bacterial cells.

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