Application of Stains-All for Demarcation of Cement Lines in Methacrylate Embedded Bone
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Biotechnic & Histochemistry
- Vol. 66 (4) , 181-184
- https://doi.org/10.3109/10520299109109966
Abstract
Cement lines provide a record of sites of past remodeling buried in the matrix of bone. A method is reported for application of Stains-all, a cationic carbocyanine dye, for demarcation of cement lines in bone. The method, which is simple, works well for both glycol methacrylate and methyl methacrylate undemineralized embedments and produces good concomitant staining of cytoplasm and nuclei of osteoblasts, osteoclasts and marrow cells.Keywords
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