Marked Caries Inhibition in the Sucrose-Challenged Rat by a Mixture of Nonnutritive Sweeteners

Abstract
The effects of acesulfame K, aspartame, cyclamate, and saccharin on cariogenesis caused by a diet containing 30% sucrose were studied in orally superinfected Cara rats. The addition of any individual sweetener had no appreciable effect on fissure caries development in this animal model. However, a 1.00:1.67:0.10 combination of acesulfame K, cyclamate, and saccharin, which was found previously to effectively inhibit acidogenesis by Streptococcus mutans and other oral microorganisms in vitro, led to a marked and highly significant reduction of fissure caries.