A simplified method for quality control of deglycerolized erythrocytes

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Abstract
Quality control to detect inadequately deglycerolized red blood cells was easily and inexpensively accomplished by suspending the deglycerolized cells in either recipient serum or normal saline in the same way in which the routine crossmatch is performed. In vitro hemolysis was readily and consistently apparent when the residual glycerol exceeded either 2.4% in saline or 2.5% in serum. In contrast to generally held beliefs, the in vivo 24-h survival of red blood cells with a residual glycerol concentration of up to 2.7% was .gtoreq. 76%, a level which is well above that usually accepted as adequate.