Specific Oligoribonucleotide Synthesis Using Primer-Independent Polynucleotide Phosphorylase
- 1 January 1975
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 5 (5-6) , 423-432
- https://doi.org/10.1080/00327487508061589
Abstract
Oligoribonucleotides of a predetermined base sequence begin-ing with adenylyl-3′ 5′-adenosine at the 5′ end have been synthesized in yields varying between 13% and 42%. The synthesis was carried out using primer-independent polynucleotide phosphory-lase from E. coli in the presence of high concentrations of primer and of sodium chloride.Keywords
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