Influence of Nitrogen and Argon Gases on Post-Thawing Motility, Laboratory Stress Tests, and Fertility of Frozen Bovine Spermatozoa

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Abstract
Flushing ampules with N2 gas was found to be superior to flushing with A or no flushing on post-thawing motility and livability at 38[degree]C in the first experiment. In the second experiment a significant difference (P<0.01) between treatments was obtained for the post-thawing motility, with mean values of 45.5 and 41.7% for the N2 flush and control, respectively. Semen treated with N2 survived considerably better (P<0.01) than the control for the livability at 39[degree]C. A slight but significant (P<0.05) increase in lactic acid production was observed at post-thawing and after the livability and 38[degree]C for semen treated with N2. Fertility based on 60- to 90-day nonreturns for 1905 first services was 74.50% for N2 flushed semen as compared to 71.8% for 1930 first services for the control. These differences did approach significance (P<0.05). A highly significant (P<0.01) simple correlation coefficient of +0.79 was found between fertility and motility after incubation at 38[degree]C.