Augmentation of the human monocyte/macrophage chemiluminescence response during short-term exposure to interferon-gamma and tumour necrosis factor-alpha

Open Access
Publisher Website
Google Scholar
Abstract
The effects of short-term (30 min) pre-incubation of human monocytes and macrophages (3-day cultured monocytes) with leucocyte-derived human interferon-gamma (IFN-γ) and recombinant human tumour necrosis factor-alpha (rTNF-α) were examined. Pre-incubation of either monocytes or macrophages with rTNF-α or IFN-γ (100 U/5 × 105 cells) augmented their respiratory burst to formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), measured by the luminol- and lucigenin-dependent chemiluminescence assay. In addition, both cell types showed a burst of respiratory activity in the presence of rTNF-α or IFN-γ only. The effects of IFN-γ were removed by adsorption with an anti- IFN-γ monoclonal antibody and those of rTNF-α were abolished by heating at 100°C, or by the addition of anti-TNF-α monoclonal antibody. The results demonstrate that both IFN-γ and rTNF-α are stimulators of monocytes and macrophages, and rapidly alter the capacity of the cells to respond to fMLP, which binds to cell surface receptors.

This publication has 39 references indexed in Scilit: