THE FRACTIONATION OF CHOLESTEROL IN BODY FLUIDS BY MEANS OF SOLVENT EXTRACTION 1

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Abstract
One ml of freshly obtained body fluid was placed in the bottom of a 10 mm drain tube and layered with 1 ml of a mixture of 1:9 absolute ethanol; ethyl ether v/v, the tube sealed and inverted 20 times. The tube was stored upright at 4[degree]C for 20 hours after which the solvent layer was analyzed. Analyses were performed on serum from 15 rats and 6 rabbits before and at 3, 6, 12 and 24 hours after feeding olive oil with or without added cholesterol; analyses were also done on intestinal lymph from 49 rats fed water, oil or oil plus cholesterol; and also on the same lymph sample after fractionation into chylomicra and non-chylomicronous layers by ultracentrifugation. The degree of extractibility of cholesterol in each case appeared to increase with the increasing lipid content of the serum lymph, or lymph fraction. These conclusions were supported by extraction of hypercholesteremic sera from bile duct obstructed rats and Triton WR-1339 injected rats. A greater fraction of the cholesterol of lymph was extracted than that from a comparable serum sample.