Effect of DNA supercoiling on in vitro transcription from the adenovirus early region 4
- 22 May 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (1) , 17-20
- https://doi.org/10.1016/0014-5793(89)80006-7
Abstract
The effect of DNA supercoiling on in vitro transcription from the early region 4 (E4) promoter of adenovirus type 5 (Ad5) has been investigated by using a cell-free transcription system that maintains supercoiled DNA templates. The supercoiled DNAs yield several-fold higher levels of E4 transcripts and more faithfully reflect the regulation of in vivo transcription than the linear DNAsKeywords
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