Analysis of the tetrahydrobiopterin synthesizing system during maturation of murine reticulocytes
- 4 February 1990
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 142 (2) , 268-271
- https://doi.org/10.1002/jcp.1041420208
Abstract
The enzymes of tetrahydrobiopterin synthesis have been studied in murine bone marow, in spleen, in erythrocytes, and in reticulocytes. Mice with chemically induced and with genetically conditioned reticulocytosis as found in the lactate dehydrogenase deficient strain (Ldh‐1c/Ldh‐1c) were used for analysis of reticulocytic enzyme activities. The activity of the biopterin synthesizing system is highest in bone marrow even though it amounts to only about 10% as compared with liver. The first enzyme of the biosynthetic pathway, GTP‐cyclohydrolase, virtually disappears during the final maturation step of reticulocytes. In contrast, the activities of 6‐pyruvoyltetrahydropterin synthase and of sepiapterin reductase of erythrocytes are only reduced to about one half of the reticulocyte level. The absence of biopterin in erythrocytes is therefore caused by the loss of the enzyme that initiates the pterin biosynthetic pathway.This publication has 29 references indexed in Scilit:
- Modulation of interleukin 2 high-affinity binding by lymphocyte-derived tetrahydrobiopterin: Pterins as potential participants in the control of interleukin 2 receptor assemblyJournal of Cellular Biochemistry, 1989
- Regulation of tetrahydrobiopterin synthesis during lectin stimulation of human peripheral blood lymphocytesFEBS Letters, 1989
- Localization of GTP cyclohydrolase i in human peripheral blood smears using a specific monoclonal antibody and an immune-alkaline phosphatase labeling techniqueBiochemical and Biophysical Research Communications, 1987
- Pteridines are produced during interleukin 2-induced T-cell proliferation and modulate transmission of this signalExperimental Cell Research, 1986
- Synthesis and interferon-γ controlled release of pteridines during activation of human peripheral blood mononuclear cellsBiochemical and Biophysical Research Communications, 1985
- Pteridine formation during lectin‐induced lymphocyte activationJournal of Cellular Biochemistry, 1985
- Two new tetrahydropterin intermediates in the adrenal medullary de novo biosynthesis of tetrahydrobiopterinBiochemical and Biophysical Research Communications, 1984
- Intermediates in the enzymic synthesis of tetrahydrobiopterin in DrosophilamelanogasterBiochemical and Biophysical Research Communications, 1984
- Tetrahydro-sepiapterin is an intermediate in tetrahydrobiopterin biosynthesisBiochemical and Biophysical Research Communications, 1983
- Erythropoietic precursors in mice with phenylhydrazine‐induced anemiaAmerican Journal of Hematology, 1976