Perfusion Preservation of Hearts for 6 to 9 Days at Room Temperature
- 20 January 1978
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 199 (4326) , 299-301
- https://doi.org/10.1126/science.619456
Abstract
The combination of a defined medium with single-pass perfusion has made possible long-term maintenance of beating rat hearts at 22 degrees C in vitro. The 6- to 9-day survival period appears to be the longest so far reported for hearts. This method provides a stable system which should be useful for investigating the role of single factors in myocardial preservation and evaluating the effects of exposure to pharmacological and toxicological agents.Keywords
This publication has 10 references indexed in Scilit:
- Effects of prolonged perfusion time on the isolated perfused rat heartToxicology and Applied Pharmacology, 1976
- 1,4-Diaminobutane (Putrescine), Spermidine, and SpermineAnnual Review of Biochemistry, 1976
- Metabolic studies of isolated canine hearts perfused at 4 °C for up to 96 hours, with assessment of viabilityCryobiology, 1975
- Twenty-four-hour preservation of the canine heartJournal of Surgical Research, 1974
- In Vitro Preservation of Canine Hearts for 24 to 28 Hours Followed by Successful Orthotopic TransplantationAnnals of Surgery, 1973
- Successful Three- to Seven-Day Preservation of Canine KidneysArchives of Surgery, 1971
- Preservation of Isolated Heart for 72 HoursBMJ, 1968
- Effect of pressure development on oxygen consumption by isolated rat heartAmerican Journal of Physiology-Legacy Content, 1967
- Accumulation of amino acids in muscle of perfused rat heart. Effect of insulinBiochemical Journal, 1965
- CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUMProceedings of the National Academy of Sciences, 1965