Blood storage XXIV: red blood cell 2,3‐DPG and ATP maintenance for six weeks in CPD‐adenine with higher phosphate, pyruvate, and dihydroxyacetone

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Abstract
The individual and collective effects of various phosphate, pyruvate and dihydroxyacetone concentrations on 2,3-DPG [diphosphoglycerated] and ATP maintenance during blood [human] storage with CPD[citrate phosphate dextrose]-adenine (0.25 mM), were studied. Phosphate concentrations ranged from 2-100 mM. Low concentrations were best for 2,3-DPG maintenance during the first 3 wk, after which there was no difference. ATP concentrations were better maintained by the highest phosphate concentrations in the 1st wk. After the 2nd wk the lower concentrations of phosphate were better. With pyruvate, 40 and 60 mM were the best for 2,3-DPG levels through 6 wk of storage. ATP concentrations were poorest with high pyruvate. Maintenance of 2,3-DPG was above half normal for 6 wk of storage in the 60, 80 and 100 mM DHA [dihydroxyacetone] preservatives. ATP concentrations were best maintained in the preservative lacking DHA. Combinations of phosphate, pyruvate and DHA in concentrations which were effective when used individually were studied. Best maintenance of 2,3-DPG (above half normal levels) for 6 wk was afforded by pyruvate, phosphate and DHA, and by pyruvate and DHA. ATP maintenance was best afforded by CPD-adenine alone and CPD-adenine with pyruvate and phosphate. Pyruvate alone maintained ATP less well and the pyruvate-DHA was worst. Intermediate in maintenance of ATP was the preservative containing pyruvate, phosphate and DHA.