HPLC Method for the Determination of Ketorolac in Human Plasma

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Abstract
A precise and accurate HPLC method for the quantification of ketorolac in human plasma is described. Ketorolac and an added internal standard (a fluoro analog of ketorolac) are extracted from acidified plasma samples and the extract is further purified. The reconstituted sample is injected onto a reverse phase HPLC column. The method has a linear concentration range of 0.010-3.00 μg of ketorolac per aliquot of plasma, using 0.1 to 1.0 mL of plasma for analysis. The quantification limit of the method is 0.010 μg/mL. The intra- and inter-assay % CVs were less than 5.5%, and the mean recoveries ranged from 96% to 109%. The absolute recovery was 92%.