STUDIES ON THE NUTRITION OF BRUCELLA MELITENSIS

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Abstract
A chemically defined growth medium was developed for strain 4247 of B. melitensis. The medium supports yields of over 125 thousand million organisms per ml. after 72-96 hrs. of incubation on a reciprocating shaker at 37[degree]C. The medium is composed of 6 amino acids (DL-alanine, 1.20 mg./ml.; L-arginine HC1, 0.77 mg./ml.; L-cystine, 0.10 mg./ml.; L_ glutamic acid, 3.90 mg./ml.; L-lysine HC1, 0.77 mg./ml.; and DL_methionine, 0.94 mg./ml.), inorganic salts (K2HPO4, 1.74 mg./ml.; MgSO4 7H2O, 0.62 mg./ml.; FeSO4 7H2O, 0.0139 mg./ml.; and MnSO4 H2O, 0.0169 mg./ml.), vits. (nicotinic acid, 2.0 [mu]g./ml. and thiamin HCl, 0.15 [mu]g./ml.) and glucose (25 mg./ml.). The glucose was sterilized separately and added to the remainder of the medium which was autoclaved after adjustment to pH 7.5 with NaOH. The medium was tested with several representative strains of brucellae (melitensis, suis and abortus) and is suitable, especially for several strains of B. melitensis.