The Amino-Acid Sequence of Trout-Testis Histone H1

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Abstract
The complete amino-acid sequence of 194 residues of trout testis histone H1 was elucidated by automatic Edman degradation of large peptides derived from specific cleavages at infrequent amino-acid residues. Trout testis histone H1 has a MW of 19,314, is only slightly microheterogeneous and possesses pentapeptide sequences related to Ala-Ala-Lys-Lys repeated 6 times in the complete sequence. Although the N-terminus of histone H1 is known to be blocked, some 10% of intact trout testis H1 contains a free N-terminal alanine residue. Three seryl residues in the C-terminal Half (97-194) of the molecule occur in the sequence Lys-Ser-Pro-Lys known to be phosphorylated in trout testis H1. The sequence was compared to the known partial sequence of rabbit thymus H1. The results are consistent with a role for histone H1 in the maintenance of the higher-order structure of chromatin.