WOUND-INDUCED PROTEINASE-INHIBITORS FROM TOMATO LEAVES .1. THE CDNA-DEDUCED PRIMARY STRUCTURE OF PRE-INHIBITOR-I AND ITS POST-TRANSLATIONAL PROCESSING

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  • 1 January 1985
    • journal article
    • research article
    • Vol. 260  (11) , 6555-6560
Abstract
A c[complementary]DNA containing the coding region for the complete amino acid sequence of wound-induced proteinase Inhibitor I from tomato leaves was constructed in the plasmid pUC9 and characterized. The open reading frame codes for a protein of 111 amino acids. This deduced amino acid sequence revealed the presence of a 42-amino acid N-terminal sequence that is not found in the native protein. This sequence appears to contain a 23-amino acid segment typical of a signal sequence followed by a 19-amino acid sequence containing 9 charged amino acids. The 42-amino acid sequence is apparently lost during maturation to the native Inhibitor I and represents 38% of the translated protein. The Inhibitor I amino acid sequence contains 71% identity with potato tuber Inhibitor I sequence and 35% identity with an inhibitor from the leech.