EFFECTS OF ALIPHATIC ALCOHOLS ON THE METABOLISM OF GLUCOSE AND FRUCTOSE IN RAT LIVER SLICES
- 1 March 1963
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 41 (3) , 793-803
- https://doi.org/10.1139/o63-090
Abstract
Ethanol, at concentrations up to 3 mM, whilst having little inhibitory effect on the production of respiratory CO2of rat liver slices, has a marked suppressing action on the formation of labelled CO2from labelled glucose. The suppression of C14O2formation by ethanol from radioactive glucose is independent of the concentration of the latter and amounts to 57% with 3 mM ethanol after 1 hour's incubation. The results are consistent with the conclusion that ethanol gives rise more rapidly than glucose to acetyl CoA and that the large suppressing action of ethanol in rat liver slices is due to isotopic dilution of labelled acetyl CoA derived from the labelled sugars with the unlabelled acetyl CoA derived from ethanol. Ethanol exercises a larger inhibition of the rate of C14O2formation from glucose-6-C14than from glucose-1-C14. The difference between the effects of ethanol on C14O2formation from glucose-1-C14and glucose-6-C14is presumably due to operation of the hexosemonophosphate shunt.The higher aliphatic alcohols have about the same diminishing effect on C14O2formation from glucose-U-C14and fructose-U-C14as does ethanol. This observation may also be a consequence of dilution of labelled acetyl CoA derived from the sugars by unlabelled acetyl CoA coming from the added alcohol. Incorporation of radioactive carbon from the labelled sugars into liver proteins and lipids is inhibited by ethanol and higher aliphatic alcohols, and the inhibitions are similar in magnitude to those of C14O2formation. These may be accounted for by isotopic dilution. The amounts of C14incorporated into lipids and proteins from radioactive glucose and fructose are small, being about 1/10th of that appearing in the CO2.The higher aliphatic alcohols suppress total CO2formation during rat liver slice respiration much more than does ethanol at equivalent concentrations.Keywords
This publication has 11 references indexed in Scilit:
- EFFECTS OF ALIPHATIC ALCOHOLS AND FATTY ACIDS ON THE METABOLISM OF ACETATE BY RAT LIVER SLICESCanadian Journal of Biochemistry and Physiology, 1961
- Alteration of the pattern of hepatic carbohydrate metabolism by propionate and butyrateArchives of Biochemistry and Biophysics, 1958
- Further studies on the inhibition of acetate metabolism by propionateBiochemical Journal, 1958
- LAEVULOSE, SUB‐EMETIC DOSES OF APOMORPHINE,1 AND OXYGEN IN THE TREATMENT OF ALCOHOL ADDICTION†British Journal of Addiction to Alcohol & Other Drugs, 1955
- Beschleunigung des Alkoholabbaus durch Fruktose beim MenschenCellular and Molecular Life Sciences, 1952
- The metabolism of fructose in liver. Isolation of fructose-1-phosphate and inorganic pyrophosphateBiochimica et Biophysica Acta, 1951
- The Effect of Alcohol on Liver Lipids and on Liver and Heart GlycogenQuarterly Journal of Studies on Alcohol, 1950
- The Glucose Tolerance of Alcohol Addicts; A Study of 303 CasesQuarterly Journal of Studies on Alcohol, 1943
- Factors Influencing the Effects of Alcohol on Blood Sugar and Liver GlycogenQuarterly Journal of Studies on Alcohol, 1941
- The Metabolism of Alcohol: A ReviewQuarterly Journal of Studies on Alcohol, 1940