A novel brain‐specific 25 kDa protein (p25) is phosphorylated by a Ser/Thr‐Pro kinase (TPK II) from tau protein kinase fractions
- 2 September 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 289 (1) , 37-43
- https://doi.org/10.1016/0014-5793(91)80903-g
Abstract
A novel brain-specific 25 kDa protein (p25) was purified from a bovine brain extract. The protein was phosphorylated by Ser/Thr-Pro kinase (TPK II) in tau protein kinase fractions at the Ser residues of Ser-Pro sequences. Using immunoblot analysis, the protein was found only in brain extracts, and was most abundant in the brain regions such as cerebrum and hippocampus, but less abundant in cerebellum, medulla oblongata and olfactory bulb. The protein was detected in rat, bovine and human brain extracts, indicating that this protein specifically exists in mammalian brain tissues.Keywords
This publication has 17 references indexed in Scilit:
- Purification and characterization of DNA ligase II from Drosophila melanogasterEuropean Journal of Biochemistry, 1990
- Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein cdc2Nature, 1989
- Resolution and purification of a neurofilament-specific kinase.Proceedings of the National Academy of Sciences, 1989
- [29] Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresisPublished by Elsevier ,1983
- [42] Detection and quantification of phosphotyrosine in proteinsPublished by Elsevier ,1983
- [1] Assays of protein kinasePublished by Elsevier ,1983
- New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugatesBiochemistry, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Purification and characterization of a protein kinase in Tetrahymena ciliaBiochimica et Biophysica Acta (BBA) - Enzymology, 1973
- Solid Phase Peptide Synthesis. I. The Synthesis of a TetrapeptideJournal of the American Chemical Society, 1963