A Study of Bentonite Flocculation Test for Measurement of Type-Specific Streptococcal Antibodies.

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Abstract
Bentonite particles 1.0-1.5 microns in diameter were sensitized with hot acid-extracted, alcohol-precipitated crude M type-specific proteins freed of group-specific polysaccharide. Using unabsorbed rabbit antisera to heat-killed cells of Group A strains of types 12, 14 and 36, flocculation titers up to 1-32 were obtained. The reactions were type-specific except for a weak, non-reciprocal cross-reaction observed between types 14 and 36. Extracts containing both group-specific C polysaccharide and M protein could not be employed for sensitization of the particles. C polysaccharide was apparently not adsorbed to the particles.