EFFECTS OF PHYSICAL-TRAINING ON ENZYMATIC-ACTIVITY OF HUMAN SKELETAL-MUSCLE

  • 1 January 1975
    • journal article
    • research article
    • Vol. 22  (6) , 496-501
Abstract
Enzymatic activities of glutamic oxalacetic transaminase (GOT), lactic dehydrogenase (LDH), malate dehydrogenase (MDH) and creatine phosphokinase (CPK) were assessed in 4 normal subjects, before and after a 12 wk training period on a cycle ergometer. Determinations were made in muscle at rest and immediately after submaximal and maximal exercise tests and in serum at rest. Muscle biopsies were obtained from the quadriceps by the needle biopsy technique. Maximal O2 uptake (.ovrhdot.VO2) increased significantly after training; the after training GOT and MDH muscle resting activities increased significantly. Correlations were found between resting muscle GOT and MDH vs. maximal .ovrhdot.VO2, and an increase of resting enzymatic serum activity after training was found for LDH and CPK and a decrease for MDH for the same period. Correlations were found between resting serum and muscle activities of LDH and between MDH and LDH serum resting values vs. maximal .ovrhdot.VO2. After training, the muscle activities of GOT and MDH at rest correlated adequately with performance. Muscle GOT activity changes were modified by training after exercise, partly explaining serum modifications. Resting serum enzyme levels did not necessarily correlate with muscle levels, and these serum levels could be an index of the achieved training.