HUMAN LYMPHOCYTES-T EXPRESSING A DEFINED T-CELL ANTIGEN RECEPTOR FAMILY SPECIFICALLY KILL THE HYBRIDOMA THAT MAKES THE ANTIRECEPTOR MONOCLONAL-ANTIBODY

  • 1 November 1986
    • journal article
    • research article
    • Vol. 59  (3) , 383-387
Abstract
Human T lymphocytes expressing a family of T-cell antigen receptors defined by a particular monoclonal antibody can be induced to grow with the Mab and recombinant IL-2. The resulting population consists largely of cells that have the phenotype T3+, T8+, T4-, and that react with the inducing anti-TcR Mab. The ability of such T cells to kill the hybridoma which produces the inducing Mab has been investigated. Specific killing is demonstrated which is inhibited by Mab to T3, TcR, and LFA-1. Further, lytic activity is rapidly lost when cultures are deprived of lymphokines. Cytolytic activity is restored by incubating cells in recombinant IL-2.