Development of a sensitive radioreceptor assay for oxyphenonium in plasma and urine
- 1 April 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 36 (4) , 235-239
- https://doi.org/10.1111/j.2042-7158.1984.tb04356.x
Abstract
A radioreceptor assay (RRA) for oxyphenonium has been developed. It is based on competition between [3H]dexetimide and oxyphenonium for binding to muscarinic receptors from calf striata. The RRA is optimized towards incubation medium and to extraction by ion pair formation with sodium picrate. At least 4 times 10−10 m of oxyphenonium is necessary to permit a reliable assay. This corresponds to a detection limit of drug of 2 ng ml−1 urine. After extraction, drug at 100 pg ml−1 of plasma can be estimated using 4 ml samples. The method is applicable to monitoring the drug and to the determination of its pharmacokinetics after therapeutic dosing. Urine levels can also be monitored.This publication has 3 references indexed in Scilit:
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- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949