Isoelectric focusing of IgG eluted from multiple sclerosis and subacute sclerosing panencephalitis brains

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Abstract
Elevated IgG distributed in oligoclonal bands is characteristically observed in the CSF of patients with multiple sclerosis (MS) or subacute sclerosing panencephalitis (SSPE). Similarly, IgG in bands has been detected in neutral saline (NS) and acid eluates of brain material from these two diseases. Isoelectric focusing (IEF) was used to compare IgG eluted from control brain, 3 plaques and a white matter pool of an MS brain, and 3 regions of an SSPE brain. A direct peroxidase-conjugated anti-human IgG staining technique was used to stain IgG exclusively and to visualize the minute amounts of IgG obtained from individual MS plaques. Eluates from individual MS plaques have distinct IgG patterns; those from separate SSPE brain areas have essentially identical IgG patterns. The identical IgG patterns in 3 areas of SSPE brain suggest a common response to the same antigen. The different IgG patterns among MS plaques suggest the following: variable response to the same MS antigen in each plaque; response to different MS antigens in different plaques; synthesis of nonsense antibodies irrelevant to the pathogenesis of MS in each plaque; some combination of the above.