Development of an isolated perfused mammalian kidney

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Abstract
This study describes the development and functional evaluation of an artificial heart-lung perfusion system in which whole blood is used to perfuse the isolated rabbit kidney. Data are presented which indicate that this preparation is capable of attaining normal or near normal function at some time during the course of the experiment in at least 40% of the last 26 experiments. Functional evaluation of the perfused kidney is made by measuring the observed renal blood flow, rate of urine flow, and extraction ratios and clearances of creatinine and PAH. In a limited number of experiments, it has been shown that in the isolated perfused kidney, there is considerable active reabsorption of glucose and sodium. Furthermore, the same response to drugs and hormones, known to affect kidney function in the intact animal (papaverine, renin and Pitressin), has been demonstrated.