Characterization and Purification of a Kallikrein from Human Pancreatic Juice and Immunological Comparison with Other Kallikreins

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Abstract
A prekallikrein was demonstrated in human pancreatic juice and the active enzyme was purified from this material. The purification procedure included filtration of Sephadex G-100 chromatography on DEAE-cellulose and affinity chromatography on trypsin-inhibitor Sepharose. The purified kallikrein appeared to be homogeneous by polyacrylamide gel electrophoresis at pH 8.3 and by immunoelectrophoresis. Human pancreatic kallikrein is immunologically different from humam plasma kallikrein and from pancreatic kallikreins of other species (hog, cat, rat and dog). Human pancreatic kallikrein has common antigenic determinants with human urinary and submandibular kallikreins but probably not with parotid kallikrein.

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