Supravital observation of in vitro basophils in immunological reactions

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Abstract
The migration velocity and morphology of basophils in vitro were examined after the addition of anti‐immunoglobulin (anti‐lgE or anti‐IgG). In atopic asthma patients with high serum IgE levels (more than 1000 i.u./ml) basophils showed increased migration velocity and showed pear‐shaped and palmate processes after the addition of anti‐lgE, but on the addition of anti‐IgG, these basophils did not show increased migration velocity or morphological changes. In intractable asthma patients with low serum IgE levels, these changes occurred with anti‐IgG but not with anti‐IgE. These in vitro findings suggest a basophil reactivity to anti‐IgE and anti‐IgG in individual patients and support our previous differentiation of asthma patients according to basophil reaction.