Occurrence of conjugated linoleic acid isomers in beef

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Abstract
The amounts of Δ9,Δ11‐conjugated linoleic acid (CLA) isomers were determined in loin‐associated fat samples of bulls (n=6) and steers (n=7) by capillary gas chromatography of fatty acid methyl ester (FAME) derivatives. The main CLA‐isomer—18:2 c9,t11—provided approximately 0.76 ± 0.15% and 0.86 ± 0.15% of total FAME in bulls and steers, respectively. No differences (P>0.05) were observed between the CLA isomer distribution of bulls (t9,c11, 0.026 ± 0.014%; c9,c11, 0.015 ± 0.008%; and t9,t11, 0.029 ± 0.003%) and steers (t9,c11, 0.027 ± 0.014%; c9,c11, 0.015 ± 0.005%; and t9,t11, 0.030 ± 0.007%).