MAPPING CDC MUTATIONS IN THE YEAST S-CEREVISIAE BY RAD52-MEDIATED CHROMOSOME LOSS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 110  (4) , 591-607
Abstract
Using the chromosome loss-mapping method of Schild and Mortimer, several new temperature-sensitive mutations were mapped that define 5 CDC genes. Modified procedures were used to facilitate mapping temperature-sensitive mutations in general, and these modifications are discussed. The mutations were assigned to specific chromosomes by chromosome loss procedures, and linkage relationships were determined subsequently by standard tetrad analysis. Four of the mutations define new loci. The 5th mutation, cdc63-1, is shown to be allelic to previously known mutations in the PRT1 gene.