Amber-mutants of plasmid mini-F defective in replication
- 1 November 1980
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 180 (1) , 201-204
- https://doi.org/10.1007/bf00267370
Abstract
Amber mutants of the mini-F plasmid pML31 were isolated with the mutagen hydroxylamine. Under non-permissive conditions amber mutants segregate and show no incorporation of label into supercoiled plasmid DNA in double-label experiments. Wild-type and one mutant of mini-F were integrated by recombinant DNA techniques into the single EcoRI site of plasmid vector pBR322. Plasmid specific proteins were labeled in minicells and analysed by SDS-PAGE. A 34,000 dalton molecular weight protein was identified to be missing in the amber mutant of plasmid mini-F.Keywords
This publication has 34 references indexed in Scilit:
- Plasmid replication functionsMolecular Genetics and Genomics, 1979
- F plasmid incompatibility and copy number genes: Their map locations and interactionsPlasmid, 1978
- Temperature-dependent and amber copy mutants of plasmid R1drd-19 in Escherichia coliPlasmid, 1978
- Isolation and characterization of the minimal fragment required for autonomous replication (“Basic replicon”) of a copy mutant (pKN102) of the antibiotic resistance factor R1Molecular Genetics and Genomics, 1978
- Conjugation proteins encoded by the F sex factorNature, 1977
- The transposon Tn1 as a probe for studying ColE1 structure and functionMolecular Genetics and Genomics, 1977
- Amber mutations in Escherichia coli essential genes: Isolation of mutants affected in the ribosomesMolecular Genetics and Genomics, 1977
- Mutagenesis of plasmid DNA with hydroxylamine: Isolation of mutants of multi-copy plasmidsMolecular Genetics and Genomics, 1976
- Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bandsFEBS Letters, 1975
- Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by FluorographyEuropean Journal of Biochemistry, 1975