Gymnoascus demonbreuniiAjello & Cheng: Evidence that it is not the perfect state ofHistoplasma capsulatumdarling

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Abstract
Strains of Gymnoascus demonbreunii Ajello & Cheng (1967c) including ATCC 18027, -18028, -18029, CBS 121.67, -122.67, -123.67, SAF-7 and M 19 were studied. All 3cultures from CBS and 2 of those from ATCC were found to produce cleistothecia of G. demonbreunii with an arthroaleuriospore asexual state. Strain ATCC 18029, reported to be a single ascospore culture of G. demonbreunii derived from Strain ATCC 18028, was found to be a pure culture which produced a Pseudoarachniotus perfect stage. Strain SAF-7 was a mixture of two species; namely, G. demonbreunii and H. capsulatum. Pure cultures of G. demonbreunii failed to convert to the yeast form, in vitro or in vivo, and failed to grow at 37°C. The fungus, however, was found to persist in a mycelial form in mouse tissues. Careful study of the asexual stage of C. demonbreunii revealed that this fungus has no taxonomic relationship to H. capsulatum. Cepas de Gymnoascus demonbreunii, Ajello y Cheng, (1967c) inclyendo ATCC 18027, -18028, -18029, CBC 121.67, -122.67, -123.67, SAF-7 y M 19 han sido estudiadas. Los tres cultivos de CBS al igual que dos de los cultivos de ATCC se encontraron producían cleistothecia G. demonbreunii con un arthroaleuriospora estado asexual. La cepa ATCC 18029, reportada ser un cultivo de una sola ascospora de G. demonbreunii derivada de la cepa ATCC 18028, se encontró ser un cultivo puro que produjo un pseudoarachniotus estado sexual. La cepa SAF-7 era una mezcla de las especies; G. demonbreunii y H. capsulatum. Cultivos puros de G. demonbreunii no se convirtieron a la forma de levadura en vitro o en vivo, y no crecieron a 37°C. El hongo, sin embargo, se encontró que persiste en forma de mycelio en los tejidos del ratón. Cuidadosos estudios del estado asexual de G. dermonbreunii revelaron que el hongo no estárelacionado con H. capsulatum.