Specific binding of poly(I) · poly(C) to the membrane of murine B lymphocyte subsets

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Abstract
Indirect immunofluorescence revealed that 13% of BALB/c and 33% of CBA spleen cells of B type carry specific binding sites at their surface for double‐stranded poly(I)·poly(C). Pretreatment of BALB/c spleen cells with anti‐mouse immunoglobulin serum increased the number of B cells capable of binding poly(I)·poly(C) indicating the existence of a second B lymphocyte subpopulation carrying masked poly(I)·poly(C)‐binding sites. Pretreatment of the cells with mitogenic doses of either lipopolysaccharide (LPS) or single‐stranded polynucleotides, e.g. poly(I) or double‐stranded poly(A)·poly(U), failed to affect binding of poly(I)·poly(C) to the cells. Poly(I)·poly(C) converts small poly(I)·poly(C)‐binding lymphocytes into lymphoblasts carrying poly(I)·poly(C)‐binding sites. Lymphoblasts derived from LPS‐stimulated cells do not carry poly(I)·poly(C)‐binding sites. Thymocytes or splenic T cells failed to bind poly(I)·poly(C). As measured by thymidine uptake, CBA mice containing a higher percentage of poly(I)·poly(C)‐binding cells, are high responder mice to poly(I)·poly(C), compared with low responder BALB/c mice.