PULMONARY INTRAVASCULAR SEQUESTRATION OF ACTIVATED NEUTROPHILS - FAILURE TO INDUCE LIGHT-MICROSCOPIC EVIDENCE OF LUNG INJURY IN RABBITS

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 108  (1) , 17-23
Abstract
Rabbits were injected i.v. with glycogen-elicited allogenic peritoneal polymorphonuclear neutrophil leukocytes (PMN) for the study of the light microscopic effects of acute and chronic sequestration of PMN in the pulmonary vascular bed. Infusion of 51Cr-labeled PMN demonstrated that .apprx. 1/2 of the cells were sequestered in the lung, with no difference observed between PMN incubated with 10% normal rabbit serum and PMN incubated with 10% zymosan-activated serum (ZAS)prior to infusion. Quantitative histologic studies demonstrated that the number of ZAS-activated PMN presented in the alveolar walls at 4 h rapidly declined over the ensuing 20 h and was back to buffer control values by 48 h. No PMN, red cells or signs of edema were visible in the alveolar spaces. In rabbits injected chronically (twice weekly for 8 wk) with 2 .times. 108 PMN (ZAS-stimulated and unstimulated), no qualitative or quantitative (mean linear intercept) evidence for damage to alveolar walls was observed. Acute and chronic pulmonary sequestration of PMN activated in vitro, infused in the absence of activated serum products, does not apparently cause light microscopic evidence of lung injury.