A Sensitive High Performance Liquid Chromatography Assay for Trospectomycin. An Aminocyclitol Antibiótic, in Human Plasma and Serum

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Abstract
This report describes a sensitive, selective and robust assay for the quantification of trospectomycin, an aminocyclitol antibiotic, in human plasma and serum. This is the first published High Performance Liquid Chramatography (HELC) bioanalytical method for a member of this class of compound. The method involves the selective solid phase extraction of 6′-n-propyl spectinomycin and the internal standard 6′-n-butyl spectinonycin from 0.5 ml of biofluid, efficient reversed phase high pressure liquid chromatography with post column oxidation, reaction with o-phthaldialdehyde and fluorescence detection. The limit of quantification from 0.5 ml of biofluid is 10 ng/ml.