A sensitive high performance liquid chromatographic assay of a new metabolite of ampicillin in man
- 1 March 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 38 (3) , 225-226
- https://doi.org/10.1111/j.2042-7158.1986.tb04551.x
Abstract
A sensitive, high-performance liquid chromatographic method has been developed for the determination of piperazine-2,5-dione, a new metabolite of ampicillin, in human urine. Piperazine-2,5-dione was separated from human urine on a C18-column using phosphate buffer-methanol (pH 3.5) as eluent. Subsequently, the effluent underwent a postcolumn reaction with mercurous chloride and was then detected at 305 nm. It was found that piperazine-2,5-dione was also excreted in the urine of a volunteer dosed with ampicillin.This publication has 3 references indexed in Scilit:
- Spin-echo 1H n.m.r. spectroscopy: a new method for studying penicillin metabolismJournal of the Chemical Society, Chemical Communications, 1984
- Structure re-assignment of a metabolite of ampicillin and amoxycillin and epimerization of their penicilloic acidsJournal of Pharmacy and Pharmacology, 1983
- Piperazinedione formation from reaction of ampicillin with carbohydrates and alcohols in aqueous solutionInternational Journal of Pharmaceutics, 1979