Efficient Production of Antibody Fragments by the Filamentous Fungus Trichoderma reesei
- 1 May 1993
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 11 (5) , 591-595
- https://doi.org/10.1038/nbt0593-591
Abstract
We have engineered the filamentous fungus Trichoderma reesei to assemble and secrete immunologically authentic engineered Fab antibody fragments into the culture medium. A major improvement in yield was achieved by fusing the heavy Fd chain to the T. reesei cellulase, CBHI. The yields of secreted, immunologically active Fab and CBHI-Fab fusion were 1 mg/l and 150 mg/l, respectively. The Fab fragment can be released from the fusion protein CBHI-Fab by an extracellular T reesei protease. There was no detectable difference in affinity for the antigen between the engineered Fab and the idiotypic antibody.This publication has 24 references indexed in Scilit:
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