Efficient cell proliferation and predominant accumulation of ɛ-globin mRNA in human leukemic K562 cells which produce mostly Hb Gower 1
- 1 May 1985
- journal article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 41 (5) , 673-675
- https://doi.org/10.1007/bf02007714
Abstract
Long-term cultures of K562(S) cells in 50–75 μM hemin allow the selection of ‘hemin-resistant’ K562 cells together with cells which proliferate efficiently while fully induced to express the human embryonic globin genes, as the hemoglobin Gower 1 (ζ2ε2) is the predominant hemoglobin produced. Our experiments demonstrate that these K562 cells accumulate mostly ɛ-globin mRNA (ɛ-globin mRNA/γ-globin mRNA=2.9) suggesting that the control of hemoglobin expression is at a pretranslational level.Keywords
This publication has 24 references indexed in Scilit:
- Human leukemia K-562 cells: induction of erythroid differentiation by 5-azacytidineCell Differentiation, 1984
- Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequencesNature, 1984
- Structural and immunological similarities between simian sarcoma virus gene product(s) and human platelet-derived growth factorNature, 1983
- The Chromosomal Basis of Human NeoplasiaScience, 1983
- CELLULAR ONCOGENES AND RETROVIRUSESAnnual Review of Biochemistry, 1983
- Desferrioxamine inhibits induced erythroid differentiation of human leukemic K-562 cellsCell Differentiation, 1983
- Predominant expression of σ and ε globin genes in human leukemia K-562(S6) variant cell lineCellular and Molecular Life Sciences, 1983
- Hemoglobin Expression in Clones of K562 Cell LineEuropean Journal of Biochemistry, 1982
- The primary structure of the human ε-globin geneCell, 1980
- K562 human leukaemic cells synthesise embryonic haemoglobin in response to haeminNature, 1979