Expression of Adhesion-Related Membrane Components in Adherent Versus Nonadherent Hamster Melanoma Cells

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Abstract
The existence of integral membrane components that are involved in cell–substratum adhesion has been postulated. Using an immunochemical approach developed in this laboratory, we provide further evidence for the role in cell–substratum adhesion of integral membrane glycoproteins within a molecular weight region of 120,000–140,000. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of material enriched approximately 100‐fold in adhesion‐related components revealed the 120,000–140,000 Mr glycoproteins in an adherent hamster melanoma cell line. These glycoproteins are greatly reduced in a non‐adherent variant. Induction of adhesion in these cells by exposure to BudR is accompanied by re‐expression of the surface adhesion antigens.