MATURATION OF RENAL ORGANIC-ACID TRANSPORT INVIVO - SUBSTRATE STIMULATION BY PENICILLIN

  • 1 January 1976
    • journal article
    • research article
    • Vol. 199  (1) , 25-31
Abstract
These experiments were designed to demonstrate that pretreatment of newborn dogs with penicillin will enhance maturation of the renal organic anion transport system in vivo. Littermate pups were injected with procaine penicillin G (300,000 IU/kg twice a day for 3 days) or saline before 2 wk of age. Animals were surgically prepared on day 16 or 17. Transport of p-aminohippurate (PAH), as an indicator of renal organic anion transport, was quantified by the following procedures: disappearance of a bolus injection from the plasma, renal extraction and rate of secretion in steady-state clearance experiments. The renal handling of inulin was measured simultaneously in all procedures for comparison. Penicillin-treated animals excreted a single i.v. bolus of PAH more rapidly than control animals, with T1/2 decreased and the constant of elimination (Ke) increased. The extraction of PAH by kidneys of penicillin-treated animals was 165% that of controls. The tubular secretion of PAH was increased in treated pups. In newborn animals the renal handling of organic anions in vivo is apparently enhanced by pretreatment with a substrate of the secretory system.

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