Characterization of an inhibitor of ribonucleic acid polymerase from the mycelial phase of Histoplasma capsulatum
- 1 May 1975
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 122 (2) , 532-537
- https://doi.org/10.1128/jb.122.2.532-537.1975
Abstract
An inhibitor of ribonucleic acid polymerases has been obtained from the mycelial phase of Histoplasma capsulatum and partially characterized. The inhibitor, called histin, was purified 200-fold by heat treatment at 100 C and electrophoresis on polyacrylamide gels. Histin moved in electrophoresis as if negatively charged; it was insensitive to treatment with ribonuclease of deoxyribonuclease but was completely digested by Pronase. Sucrose gradient centrifugation suggests a molecular weight of 24,000. The possibility of a regulatory role for histin in the life cycle of H. capsulatum is discussed.Keywords
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